DnaA boxes in the P1 plasmid origin: the effect of their position on the directionality of replication and plasmid copy number
Identifieur interne : 003573 ( Main/Exploration ); précédent : 003572; suivant : 003574DnaA boxes in the P1 plasmid origin: the effect of their position on the directionality of replication and plasmid copy number
Auteurs : Kyusung Park [États-Unis] ; Dhruba K. Chattoraj [États-Unis]Source :
- Journal of Molecular Biology [ 0022-2836 ] ; 2001.
English descriptors
- KwdEn :
- Teeft :
- Acad, Anking sequence, Appropriate antibiotics, Bacteriol, Bamhi, Band intensities, Beckman, Bidirectional, Bidirectional replication, Binding protein, Biol, Chattoraj, Chem, Chimeric plasmids, Coli, Consensus dnaa, Consensus dnaa boxes, Copy number, Copy numbers, Dnaa, Dnaa boxes, Dnaa functions, Dnaa protein, Ecori, Escherichia, Escherichia coli, Form molecules, Helicase, Helicase loading, Hindiii, Hindiii site, Initiation site, Iterons, Kmno4, Linearized, Migration patterns, Minip1, Minip1 plasmid, Minip1 plasmids, Natl, Natl acad, Open region, Opening signal, Oric, Origin opening, P1ori, Plasmid, Plasmid copy number, Plasmid origin, Plasmid replication, Plasmid replication figure, Proc, Psti fragment, Pvui, Pvui site, Repa, Replication, Replication forks, Replication initiation, Replication intermediates, Replication origin, Resultant plasmid, Styi, Transcription, Unpublished results.
Abstract
Abstract: The DnaA protein is essential for initiation of DNA replication in a wide variety of bacterial and plasmid replicons. The replication origin in these replicons invariably contains specific binding sites for the protein, called DnaA boxes. Plasmid P1 contains a set of DnaA boxes at each end of its origin but can function with either one of the sets. Here we report that the location of origin-opening, initiation site of replication forks and directionality of replication do not change whether the boxes are present at both or at one of the ends of the origin. Replication was bidirectional in all cases. These results imply that DnaA functions similarly from the two ends of the origin. However, origins with DnaA boxes proximal to the origin-opening location opened more efficiently and maintained plasmids at higher copy numbers. Origins with the distal set were inactive unless the adjacent P1 DNA sequences beyond the boxes were included. At either end, phasing of the boxes with respect to the remainder of the origin influenced the copy number. Thus, although the boxes can be at either end, their precise context is critical for efficient origin function.
Url:
DOI: 10.1006/jmbi.2001.4741
Affiliations:
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The replication origin in these replicons invariably contains specific binding sites for the protein, called DnaA boxes. Plasmid P1 contains a set of DnaA boxes at each end of its origin but can function with either one of the sets. Here we report that the location of origin-opening, initiation site of replication forks and directionality of replication do not change whether the boxes are present at both or at one of the ends of the origin. Replication was bidirectional in all cases. These results imply that DnaA functions similarly from the two ends of the origin. However, origins with DnaA boxes proximal to the origin-opening location opened more efficiently and maintained plasmids at higher copy numbers. Origins with the distal set were inactive unless the adjacent P1 DNA sequences beyond the boxes were included. At either end, phasing of the boxes with respect to the remainder of the origin influenced the copy number. Thus, although the boxes can be at either end, their precise context is critical for efficient origin function.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Park, Kyusung" sort="Park, Kyusung" uniqKey="Park K" first="Kyusung" last="Park">Kyusung Park</name></noRegion><name sortKey="Chattoraj, Dhruba K" sort="Chattoraj, Dhruba K" uniqKey="Chattoraj D" first="Dhruba K" last="Chattoraj">Dhruba K. Chattoraj</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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